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Objective To analyze the levels of IgE,TNF-α and FeNO in children with acute attack of bronchial asthma and their correlation with the severity of bronchial asthma, so as to provide theoretical basis for clinical evaluation of bronchial asthma. Methods A total of 547 children with acute bronchial asthma treated in Chengdu Women and Children's Central Hospital from January 2020 to December 2020 were selected and divided into mild group (n=287), moderate group (n=186) and severe group (n=74) according to the severity of their disease. All the children's symptoms were controlled after treatment. The serum IgE, TNF-α and FeNO levels in the experimental group were compared between the acute attack stage and the clinical control stage. Spearman correlation analysis was used to analyze the correlation between the serum IgE, TNF-α and FeNO levels and the severity of the disease. ROC curve of children with bronchial asthma was drawn to analyze the differential diagnosis value of serum IgE, TNF-α and FeNO levels in children with acute bronchial asthma. Results The levels of IgE, TNF-α and FeNO in acute stage were significantly higher than those in clinical control stage (P<0.05). The levels of serum IgE, TNF-α and FeNO in severe group were higher than those in mild and moderate groups significantly (P<0.05). The levels of serum IgE, TNF-α and FeNO in moderate group were higher than those in mild group significantly (P<0.05). Spearman correlation analysis showed that serum IgE, TNF-α and FeNO water were positively correlated with the severity of bronchial asthma (r=0.419 , 0.438 , 0.502 , P<0.05). ROC curve analysis showed that the AUC, sensitivity, accuracy and specificity of serum IgE, TNF-α and FeNO levels combined in diagnosing the severity of bronchial asthma in patients with acute attack was 0.938 (95% CI: 0.912-0.982 ), 83.47%, 92.06%, 94.28%. Conclusion The level of serum IgE, TNF-α and FeNO in children with acute attack of bronchial asthma is closely related to the severity of the disease, and combined detection of the three can be used to evaluate the severity of the disease in children.
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Objective To investigate the effect of human papilloma virus (HPV) DNA detection on cervical cancer and its precancerous lesions screening.Methods A total of 2 852 patients participated in cervical screening in Sichuan Provincial People's Hospital from January 2014 to December 2016 were enrolled.The HPV DNA testing and pathological biopsy were conducted in all patients,and the pathological examination results were defined as the gold standard.The influences of age,regions of residence (rural and urban) and obesity on the positive rate of HPV DNA were analysed.The results of HPV DNA testing in different pathological types were compared,and the sensitivity,specificity,positive predictive value,negative predictive value of of HPV DNA testing were analysed,as well.Results The positive rate of HPV DNA in the 18-＜35 age group was significantly higher than that in the 35-＜50 age group and 50-58 age group,the differences were statistically significant (P＜0.05).There was no statistically significant difference in positive rate of HPV DNA between the 35-＜50 age group and 50-58 age group (P＞0.05).The positive rate of HPV DNA of patients in urban areas was significantly lower than that of those patients in rural areas (P＜0.05),while no statistically significant difference was found in the positive rate of HPV DNA between patients with normal weight and patients whose weight exceeded the standard (P＞0.05).Among patients with positive HPV DNA testing results,the detection rate of cervical cancer and its precancerous lesions based on pathological examination was 1.60 ％ and 28.60 ％,respectively.The sensitivity,specificity,positive predictive value,negative predictive value and missed diagnosis rate of HPV DNA testing was 87.79％,86.98 ％,30.20 ％,99.11％ and 12.21％,respectively.Conclusion HPV DNA detection has a high sensitivity and specificity in cervical screening.
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Objective To understand the epidemiological characteristics of IgM antibodies against 9 kinds of respiratory patho gens of children in Chengdu area so as to provide evidence for clinically effective prevention and treatment.Methods The IgM antibodies of 9 pathogens in the serum were detected by using indirect immunofluorescence assay.The positive rate of IgM antibodies and its seasonal distribution were observed.Results Among 20 647 cases,there were total 5 623 positive cases of IgM antibodies against 9 kinds of respiratory pathogens and the positive rate was 27.23％;The positive rate of mycoplasma pneumoniae(MP) was highest,followed by influenza B virus (INFBV) and parainfluenza virus (PIV) with the detection rate of 15.95％,6.27％ and 4.35 ％,respectively.Positive rates of MP,INFBV and PIV of girls were higher than those of boys,and the difference was statistically significant(P＜0.05);The distribution differences of most pathogens infection were statistically significant in different age groups(P＜0.05);In addition,infection of most them displayed a seasonal distribution,and the difference were statistically significant(P＜0.05).Conclusion MP and INFBV are the main pathogens in respiratory tract infection of children in Chengdu area,and the infection rates of MP,INFBV and PIV among girls more higher than that among boys,and the incidence of the infections caused by the respiratory tract pathogens vary in the age and seasons.
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Objective To investigate the effect of human papilloma virus (HPV) DNA detection on cervical cancer and its precancerous lesions screening.Methods A total of 2 852 patients participated in cervical screening in Sichuan Provincial People's Hospital from January 2014 to December 2016 were enrolled.The HPV DNA testing and pathological biopsy were conducted in all patients,and the pathological examination results were defined as the gold standard.The influences of age,regions of residence (rural and urban) and obesity on the positive rate of HPV DNA were analysed.The results of HPV DNA testing in different pathological types were compared,and the sensitivity,specificity,positive predictive value,negative predictive value of of HPV DNA testing were analysed,as well.Results The positive rate of HPV DNA in the 18-＜35 age group was significantly higher than that in the 35-＜50 age group and 50-58 age group,the differences were statistically significant (P＜0.05).There was no statistically significant difference in positive rate of HPV DNA between the 35-＜50 age group and 50-58 age group (P＞0.05).The positive rate of HPV DNA of patients in urban areas was significantly lower than that of those patients in rural areas (P＜0.05),while no statistically significant difference was found in the positive rate of HPV DNA between patients with normal weight and patients whose weight exceeded the standard (P＞0.05).Among patients with positive HPV DNA testing results,the detection rate of cervical cancer and its precancerous lesions based on pathological examination was 1.60 ％ and 28.60 ％,respectively.The sensitivity,specificity,positive predictive value,negative predictive value and missed diagnosis rate of HPV DNA testing was 87.79％,86.98 ％,30.20 ％,99.11％ and 12.21％,respectively.Conclusion HPV DNA detection has a high sensitivity and specificity in cervical screening.
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Objective To understand the epidemiological characteristics of IgM antibodies against 9 kinds of respiratory patho gens of children in Chengdu area so as to provide evidence for clinically effective prevention and treatment.Methods The IgM antibodies of 9 pathogens in the serum were detected by using indirect immunofluorescence assay.The positive rate of IgM antibodies and its seasonal distribution were observed.Results Among 20 647 cases,there were total 5 623 positive cases of IgM antibodies against 9 kinds of respiratory pathogens and the positive rate was 27.23％;The positive rate of mycoplasma pneumoniae(MP) was highest,followed by influenza B virus (INFBV) and parainfluenza virus (PIV) with the detection rate of 15.95％,6.27％ and 4.35 ％,respectively.Positive rates of MP,INFBV and PIV of girls were higher than those of boys,and the difference was statistically significant(P＜0.05);The distribution differences of most pathogens infection were statistically significant in different age groups(P＜0.05);In addition,infection of most them displayed a seasonal distribution,and the difference were statistically significant(P＜0.05).Conclusion MP and INFBV are the main pathogens in respiratory tract infection of children in Chengdu area,and the infection rates of MP,INFBV and PIV among girls more higher than that among boys,and the incidence of the infections caused by the respiratory tract pathogens vary in the age and seasons.
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Objective To summarize the cause and the level change of laboratory indicators in children with hemphagocytic lym‐phohistiocytosis(HLH) .Methods Retrospectively analyze the cause and the diagnose features of the 13 HLH patients .Results The first symptom of the desease was hyperpyrexia as well as the hepatosplenomegaly and the lymphadenectasis .Then it went with the peripheral cytopenia in two or three lineages ,different degrees of the liver dysfunction and the coagulation abnormalities .The number of histiocytes increased and hemophagocytic macrophages were found in bone marrow .It also showed that the elevated ser‐um triglycerides ,serum ferritin ,lactate dehydrogenase and glutamic‐pyruvic transaminase and decreased fibrinogen in laboratory findings .Conclusion Cytopenia(in two or three lineages ,especially the platelet) ,the elevated serum triglycerides ,serum ferritin , lactate dehydrogenase ,glutamic‐pyruvic transaminase ,decreased fibrinogen and the hemophagocytic macrophages in bone marrow (≥1% ) are important indicators in hemphagocytic lymphohistiocytosis diagnosis ,which could help us know the change of the HLH laboratory features better and diagnose the disease correctly .
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Objective To evaluate the performance verification of Mindary BC5390 hematology analyzer .Methods According to requirement of CLSI documents ,the precision ,accuracy ,linearity ,carryover were evaluated .Results The background counts all met the designed requirements of manufacturer .Precision ,accuracy ,linearity of the WBC ,RBC ,PLT ,Hb ,HCT ,MCV were good and the contamination carrying rates were low .Except for basophils ,the classification of leukocyte was well correlated with the artificial microscopy classification(r2 >0 .95) .Conclusion The BC5390 is a ideal automated hematology analyzer which has high precision and accuracy ,wide linearity ,low contamination carrying rate ,and it can be used for the blood samples analysis .
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Objective To investigate the diagnostic value and significance of myocardial injury markers in neonates.Methods A retrospective analysis of 838 cases of myocardial injury markers in neonates,the total positive rate of hs -cTnI and the positive rates of hs -cTnI(hypersensitivity cardiac troponin I)in different kinds of neonatal disease were calculated,the levels of abnormalities consistent rate in hs -cTnI and CK -MBmass(creatine kinase MB mass)were compared with hs -cTnI and MYO(Myoglobin).Results The total positive rate of hs -cTnI was 40.33% in the 838 neonates.The highest incidence of myocardial injury was neonatal sepsis (57.14%),followed by neonatal pulmonary hemorrhage (55.56%)and neonatal convulsions (54.55%).The abnormalities consistent rate of hs -cTnI and CK -MBmass was better than hs -cTnI and MYO (85.50% vs 28.11%,χ2 =226.9,P <0.05). Conclusion Neonatal hospitalized children often complicated by myocardial injury;As a biochemical myocardial injury marker,the hs -cTnI detection is important for early detection of myocardial injury,it should be recommend as routine test items;CK -MBmass has better correlation with hs -cTnI than MYO,which can provide guide for doctors to interpret the data of myocardial injury markers.
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Objective To review the clinical and laboratory characteristics of the hemohemocytopenia diseases in children ,in or-der to improve the understanding of the related diseases and the level of diagnosis and treatment .Methods A total of 217 children with hemocytopenia diseases with one ,two or three series of cell reducing ,and the degree of blood cells reduce with anemia and common diseases in children .Results In 185 cases with the hemocytopenia diseases ,1 series reduce were mainly in idiopathic thrombocytopenic purpura(ITP ,37 .2% )、thrombocytopenia (19 .8% ) ,granulocytopenia or agranulocytosis (9 .1% ) .2 series re-duces mainly in acute leukemia (43 .6% ) ,the IT P (21 .7% ) and aplastic anemia (6 .5% ) .3 series reduce mainly in acute leukemia (38 .9% ) and aplastic anemia(33 .6% ) .Common cause of anemia in children with 180 cases of hemohemocytopenia diseases were a-cute leukemia (20 .0% ) ,the ITP(19 .4% ) and no clinical diagnosis of anemia (16 .1% ) .Conclusion After analyzing the character-istics of the patient′s clinical and laboratory in 217 cases of hemocytopenia diseases ,improve the clinical blood disease related knowl-edge ,help understand the laboratory data and proportion .
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Objective Through analyzing and summarizing the main pathogens of bacterial infections of children in Sichuan Province and trends of drug resistance in 2013 to provide a reference for the clinical use of antibiotics.Methods The pediatric pathogen were collected by member of Sichuan province in china antimicrobial resistance surveillance system.Results A total of 22 470 clinical bacterial were isolated from the members,in which Staphylococci,Escherichia coli,Streptococcus pneumoniae,Kleb-siella pneumoniae,Hemophilus influenza,Moraxella catarrhalis were the most common Bacteria.The resistance rates of Staphylo-coccus aureus and Coagulase-negative staphylococci to oxacillin were 1 7.7% and 71.1%.4 strains of Staphylococcus aureus and 20 strains of coagulase-negative staphylococci were teicoplanin resistance.Escherichia coli and Klebsiella pneumoniae were clearly re-sistant to the third generation cephalosporins,ampicillin and ampicillin/sulbactam,with the exception of ceftazidime.Carbapenems remained highly active against all the target bacteria.The resistance rate of Streptococcus pneumoniae to Penicillin was 3.9% .All antibiotics excepted cotrimoxazole remained highly active against the haemophilus influenzae.All antibiotics except macrolide anti-biotics remained highly active against the moraxella catarrhalis.Conclusion Penicillin-insensitive Streptococcus pneumoniae,mac-rolides-resistant gram-positive cocci,cephalosporin-resistant Enterobacteriaceae ,Oxacillin-resistance coagulase-negative staphylo-cocci were revealed to be the most serious problems in terms of bacteria resistance for children in Sichuan province.
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Objective To investigate the diagnostic values of vaginitis five of the joint inspection kit combined with microscopic examination for common vaginal disease.Methods The vaginal secretions samples from 4 114 outpatients were tested with LTS-V400 vaginitis five of the joint inspection kit and microscopic examination.The examination results of age groups were analyzed and compared.Results In all 4 114 cases of samples,the positive rate of trichomonad(1.95%)was significantly lower than that of fun-gi(4.74%),P 40-50-age group,which were 1.95%,6.10%,14.15%,10.24% and 46.34%,respectively. The positive rates of H2 O2 ,pH >4.5 and pH 50 age group,which were 85.43%,86.09%,and 0.66%,respectively.The positive rates of trichomonad,SNA,LE,H2 O2 ,pH>4.5 and pH<3.8 were statistically different a-mong the age groups(P <0.05 ).Conclusion Vaginitis five of the joint inspection kit combined with microscopic examination is suitable for the diagnosis of trichomonas vaginitis,mouldsex vaginitis,and bacterial vaginal disease .
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BACKGROUND: Polypyrrole (PPy) has been widely applied in biomedical fields due to its special electronic property. Past 20 years, there are an increasing number of studies on the application of PPy as a potentially electrically addressable tissue/cell support substrate for tissue/cell regeneration.OBJECTIVE: To overall review the application of PPy in tissue engineering field, and to provide a new approach for the research and development of medical biomaterials.METHODS: Pubmed and Chinese biomedicine literature database were searched using key word of "polypyrrole" for documents published between 1990 and 2010. Literatures related to application of PPy in tissue engineering field were included, and the repetitive articles were excluded.RESULTS AND CONCLUSION: Totally 762 papers were searched by computer, according to the inclusive and exclusive criteria, 51 literature were reviewed. Currently, PPy has been widely used in the fields of cardiovascular tissue engineering, bone and muscle tissue engineering, as well as skin tissue engineering. Application of PPy and PPy-based biomaterials hold great potential in development of novel biomedical materials applied in tissue engineering due to their versatile functionality and superior biocompatibility.
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Objective To appraise the values of combined determination of cTnI,MYO and CK-MB mass in the diagnosis of viral myocarditis.Methods The myocardial injury markers(cTnI,MYO and CK-MB mass)and myocardial enzyme(LDH,AST,CK and ?-HBDH)of 61 patients with viral myocarditis(VMC),74 patients with non-viral myocarditis(NVMC)and 46 healthy subjects were determined,respectively,by microparticle chemiluminescent immunoassay and continuous monitoring assay.Diagnostic efficiency was calculated by matrix decision method.Results The serum levels of cTnI,MYO and CK-MB mass of the patients in VMC group were obviously higher than that of the patients in NVMC group(0.46?0.21 vs 0.06?0.04?g/L,98.7?38.2 vs 39.2?26.8?g/L and 6.1?4.2 vs 2.2?1.7?g/L,respectively)as well as that of healthy subjects(0.46?0.21 vs 0.07?0.04?g/L,98.7?38.2 vs 36.5?24.7?g/L and 6.1?4.2 vs 2.1?1.5?g/L,respectively,P0.05).For preliminary diagnosis of VMC,the SE of MYO is highest,but the SP of cTnI and CK-MB mass were better choice.Restoration of MYO was earliest while cTnI was latest during convalescence of the patients.Conclusion Combined and uninterrupted determination of the myocardial injury markers can provide better diagnostic accuracy.
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Objective: Interleukin-10 (IL-10) is an anti-inflammatory helper T cell type 2 (Th2) cytokine that modulates Th1-type cytokine production. The purpose of present study was to detect the expression and changes of IL-10 in the rats heart transplantation, as determine the role of IL-10 in the acute rejection of rats heart transplantation. Methods: Hearts from DA rats were transplanted into the necks of Wistar recipients. Allografts was divided into five groups: group A (control, n=12), group B (DST treated, n=12), group C (IL-2 Mab treated, n=12), group D (IL-4 Mab treated, n=12), group E (IL-2 Mab treated, n=12). The local expression of IL-10 was analyzed at day 1,3,5,7,9,11,14 by reversed transcripition polymerase chain reaction (RT-PCR). Results: Allografts survival time was (8.3±1.7) days, (29.2±7.1) days, (26.4±5.7) days, (10.2±1.9) days and (55.0±10.6) days (P＜0.05， P＜0.01), respectively. When compared with group A, the level of IL-10 was upregulated in group B, group C and group E, especially in group E. The upregulation of IL-10 was related to the grafts survival time. Conclusion: IL-10 plays an important role in the development of acute transplantational rejection, suggests that Th-2 type deviation may contribute to the prolongation of graft survival.
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Four hybridomas which produce the monoclonal antibodies against C-reactive protein(CRP)were reported.ELISA was used to screen the hybridomas secreting monoclonal antibodies to CRP,and subcloning was carried out by limited dilution. These two sets of monoclonal antibodies by GHB7 and GHB10 were purified from ascites by the use of QAE-Sephadex A50.The purity of these two sets of antibodies which was tested by SDS gel elctrophoresis,showed one band only.Isoelectrefocus- ing was performed and showed that IP of the two types of antibodies is about pH 5.0-5.5,The subclass of four antibodies was IgG2b,IgG2a IgA,IgM respectively. The experimental conditions of preparation of hybridomas were discussed.
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To investigate the cellular immunity abnormalities of patients with rheumatoid arthritis(RA), peripheral blood lymphocytes subpopulations, interleukin 2 (IL-2) production and natural killer (NK) cell activity were determined in 9 patients with RA. The results showed that there were a remarkable decrease in NK cell activity and poor response to IL-2 stimlulation. IL-2 production and.the expression of membrane-bound IL-2 receptor were increased in RA patients. The percentage of T4 positive cells and the ratio of T4/T8 were also increased in patients with RA. The results indicate that there are severe cellular immunity abnormalities in patients with RA.